Innovations

TAPE: Targeted Accelerated Protein Evolution

Within a selected gene, TAPE is an in vitro technology allowing to rapidly & simultaneously introduce random mutations in any number of selected regions, while conserving intact any number of coding domains.

Mutated and untouched regions are preliminary defined, as well as the densities of required mutations.

TAPE is applicable to any known gene type, for creating variability in proteins (such as phage homing fibrils), enzymes, antibodies (including MAB)..., while preserving macromolecule function and or activity.

• TAPE leads to creating analogues of bio macromolecules, while preserving their activities.
• Subsequent analogues can be target tested to detect those with better inhibition or activation parameters.

Ab-ACCUS: Phage Engineering

A recombination technology allowing the rapid & efficient production of T4 phage banks in which each individual differs from all others for at least one component of its host-targeting system.

• The phage host-targeting proteins are stochastically engineered to encode hypervariable regions interspersed between constant domains.
• Each engineering step generates thousands of variants for a given protein.
• When several proteins are being thus manipulated, the number of variants nears practical infinity.
• The host-range resulting from each engineering manipulation is unknown.
• The combination of TAPE and Ab-ACCUS leads to millions of phage variotypes, with modified host range profiles and the capacity to infect a variety of bacterial serotypes within a single species, as well as different bacterial species such as Escherichia spp., Pseudomonas spp. or Yersinia spp...

 RIPh :  Inhibition of Phages

• A process for stopping phage replication in host, once DNA is injected into bacteria.
• A reversible system to restart phage DNA replication and lytic cycle, as needed.